Elimination of Chrysanthemum stunt viroid (CSVd) from meristem tip culture combined with prolonged cold treatment
نویسندگان
چکیده
منابع مشابه
Low Temperature Treatment Affects Concentration and Distribution of Chrysanthemum Stunt Viroid in Argyranthemum
Chrysanthemum stunt viroid (CSVd) can infect Argyranthemum and cause serious economic loss. Low temperature treatment combined with meristem culture has been applied to eradicate viroids from their hosts, but without success in eliminating CSVd from diseased Argyranthemum. The objectives of this work were to investigate (1) the effect of low temperature treatment combined with meristem culture ...
متن کاملSequence variability of Chrysanthemum stunt viroid in different chrysanthemum cultivars
Viroids are the smallest infectious agents, and their genomes consist of a short single strand of RNA that does not encode any protein. Chrysanthemum stunt viroid (CSVd), a member of the family Pospiviroidae, causes chrysanthemum stunt disease. Here, we report the genomic variations of CSVd to understand the sequence variability of CSVd in different chrysanthemum cultivars. We randomly sampled ...
متن کاملelimination of bean yellow mosaic virus through thermotherapy combined with meristem-tip culture in gladiolus corms
bean yellow mosaic virus (bymv, potyvirus, potyviridae) causes serious disease in gladiolus spp. in this work, the possibility of obtaining bymv free plant material from virus infected gladiolus corms was studied. thermotherapy, meristem-tip culture and combination of both techniques on infected corms/meristem-tip explants (0.5–1 mm in length) resulted in bymv elimination up to 15.38, 78.04 and...
متن کاملComplete Genome Sequences of Chrysanthemum Stunt Viroid from a Single Chrysanthemum Cultivar
The chrysanthemum stunt viroid (CSVd), a member of the genus Pospiviroid with a single circular RNA genome, infects many chrysanthemum species. Here, we report 25 complete genome sequences of CSVd in a single chrysanthemum cultivar, revealing 20 variants.
متن کاملChrysanthemum stunt viroid: primary sequence and secondary structure.
The sequence of the 356 nucleotide residues of chrysanthemum stunt viroid (CSV) has been determined. Overlapping linear viroid fragments were obtained by partial ribonuclease digestion, radiolabelled in vitro at their 5'-ends, and sequenced using partial enzymic cleavage methods. Of the CSV sequence, 69% is contained in the published sequence of potato spindle tuber viroid (PSTV). Differences i...
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ژورنال
عنوان ژورنال: Horticulture, Environment, and Biotechnology
سال: 2013
ISSN: 2211-3452,2211-3460
DOI: 10.1007/s13580-013-0141-8